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Acute and subchronic oral toxicity of Coriolus versicolor standardized water extract in Sprague-Dawley rats

Hor, Sook Yee, Ahmad, Mariam, Farsi, Elham, Lim, Chung Pin, Asmawi, Mohd. Zaini, Yam, Mun Fei
Journal of ethnopharmacology 2011 v.137 no.3 pp. 1067-1076
Coriolus versicolor, acute toxicity, adverse effects, females, histopathology, lethal dose, males, medicinal plants, mortality, no observed adverse effect level, oral administration, patients, rats, subchronic toxicity, tissue weight, toxicity testing
ETHNOPHARMACOLOGICAL RELEVANCE: Coriolus versicolor, which is known as Yun Zhi, is one of the commonly used Chinese medicinal herbs. Recent studies have demonstrated its antitumor activities on cancer cells which led to its widespread use in cancer patient. However, little toxicological information is available regarding its safety. The present study evaluated the potential toxicity of Coriolus versicolor standardized water extract after acute and subchronic administration in rats. MATERIALS AND METHODS: In acute toxicity study, Coriolus versicolor water extract was administered by oral gavage to Sprague-Dawley (SD) rats (6 males, 6 females) at single doses of varying concentrations 1250, 2500 and 5000mg/kg. In subchronic toxicity study, the extract was administered orally at doses of 1250, 2500 and 5000mg/kg/day for 28 days to male and female SD rats respectively. General behavior, adverse effects and mortality were determined throughout the experimental period. Haematological and biochemical parameters, relative organ weights and histopathological were evaluated at the end of the experiment. RESULTS: There were no mortality and signs of toxicity in acute and subchronic toxicity studies. In the single dose acute toxicity and repeated dose 28-day subchronic toxicity studies, there were no significant difference in body weight, relative organ weight, haematological parameters, clinical chemistry, gross pathology and histopathology between treatment and control groups. CONCLUSIONS: Coriolus versicolor water extract did not cause remarkable adverse effect in SD rats. The oral lethal dose of Coriolus versicolor water extract is more than 5000mg/kg and no-observed-adverse-effect level (NOAEL) of the extract for both male and female rats is 5000mg/kg per day for 28 days.