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Chicken IL-17F: Identification and comparative expression analysis in Eimeria-infected chickens
- Kim, Woo H., Jeong, Jipseol, Park, Ae R., Yim, Dongjean, Kim, Yong-Hwan, Kim, Kwang D., Chang, Hong H., Lillehoj, Hyun S., Lee, Byung-Hyung, Min, Wongi
- Developmental and Comparative Immunology 2012 v.38 no.3 pp. 401
- Eimeria maxima, Eimeria tenella, amino acid sequences, bioactive properties, chickens, coccidiosis, complementary DNA, fibroblasts, gene expression regulation, homeostasis, interleukins, large intestine, liver, lymphocytes, mammals, recombinant proteins, sequence homology, small intestine, splenocytes
- Interleukin-17F (IL-17F) is a proinflammatory cytokine, which plays an important role in gut homeostasis. A full-length chicken IL-17F (chIL-17F) cDNA with a 510-bp coding region was identified from ConA-activated chicken splenic lymphocytes. ChIL-17F shares 53% amino acid sequence identity with the previously described chicken IL-17 (chIL-17A) and 38-43% with mammalian homologues. The locus harboring chIL-17 and chIL-17F displayed inverted order compared to those of mammals. ChIL-17F transcript expression was high in lymphoblast cell line CU205 and at moderate levels in small and large intestines and liver. ChIL-17F and chIL-17 expression profiles were examined by quantitative real-time RT-PCR in mitogen-stimulated splenic lymphocytes and intestinal areas affected by Eimeria maxima and Eimeria tenella infections. Expression levels of chIL-17F, like chIL-17, were elevated in mitogen-activated splenic lymphocytes. ChIL-17F, but not chIL-17, expression was upregulated in intestinal tissues affected by E. maxima and E. tenella infections. Recombinant chIL-17F biological activities were similar to that of chIL-17 in primary chicken embryonic fibroblasts. These results suggest that chIL-17F is a unique member of the IL-17 family of cytokines.