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Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application
- Zhao, Fuhua, Wang, Qiao, Dong, Jianjun, Xian, Mo, Yu, Junhong, Yin, Hua, Chang, Zongming, Mu, Xindong, Hou, Tonggang, Wang, Jianxun
- Process biochemistry 2017 v.57 pp. 87-94
- alginates, beers, brewing industry, coprecipitation, corolla, diacetyl, fermentation, immobilized enzymes, microbeads, nanoflowers, off flavors, tricalcium phosphate
- In this work, novel nanoflower/alginate microbeads were synthesized to immobilize α-acetolactate decarboxylase (ALDC) with a facile approach. Typically, ALDC was first coprecipitated with Ca3(PO4)2 to form enzyme-inorganic hybrid nanoflowers (Ca3(PO4)2-ALDC), and then the nanoflowers containing micrometre-sized particles and nanoscale flower-like petals were entrapped in alginate gel beads (Ca3(PO4)2-ALDC) @Alg). Compared with free ALDC and Ca3(PO4)2-ALDC nanoflowers, the microbeads performed greatly improved recyclability and stability, meanwhile, retained 98% of activity compared with free ALDC. Moreover, the immobilized enzyme can be used in a 300L beer fermentation tank to prevent the formation of diacetyl, solve the problem of beer off-flavor and short the beer maturation time, thus exhibiting a great potential application in beer brewing industry.