U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria

Author:
Imane Laraba, Houda Boureghda, Nora Abdallah, Oussama Bouaicha, Friday Obanor, Antonio Moretti, David M. Geiser, Hye-Seon Kim, Susan P. McCormick, Robert H. Proctor, Amy C. Kelly, Todd J. Ward, Kerry O'Donnell
Source:
Fungal genetics and biology 2017 v.103 pp. 34-41
ISSN:
1087-1845
Subject:
3-acetyldeoxynivalenol, Bayesian theory, Fusarium culmorum, chemotypes, crown rot, genetic markers, head blight, mating types, microsatellite repeats, nivalenol, pathogens, polymerase chain reaction, population structure, surveys, sympatry, wheat, Algeria, Australia, Italy, United States
Abstract:
Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR=110, n FHB=30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (n=27), Australia (n=30) and the United States (n=28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where two-thirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8% 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77%) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89% of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.
Agid:
5667953
Handle:
10113/5667953