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Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria
- Imane Laraba, Houda Boureghda, Nora Abdallah, Oussama Bouaicha, Friday Obanor, Antonio Moretti, David M. Geiser, Hye-Seon Kim, Susan P. McCormick, Robert H. Proctor, Amy C. Kelly, Todd J. Ward, Kerry O'Donnell
- Fungal genetics and biology 2017 v.103 pp. 34-41
- 3-acetyldeoxynivalenol, Bayesian theory, Fusarium culmorum, chemotypes, crown rot, genetic markers, head blight, mating types, microsatellite repeats, nivalenol, pathogens, polymerase chain reaction, population structure, surveys, sympatry, wheat, Algeria, Australia, Italy, United States
- Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR=110, n FHB=30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (n=27), Australia (n=30) and the United States (n=28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where two-thirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8% 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77%) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89% of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.