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Antioxidant and cytotoxic lignans from the roots of Bupleurum chinense

Author:
Li, Dan-Qi, Wang, Di, Zhou, Le, Li, Ling-Zhi, Liu, Qing-Bo, Wu, Ying-Ying, Yang, Jing-Yu, Song, Shao-Jiang, Wu, Chun-Fu
Source:
Journal of Asian natural products research 2017 v.19 no.5 pp. 519-527
ISSN:
1477-2213
Subject:
2,2-diphenyl-1-picrylhydrazyl, Bupleurum chinense, antioxidant activity, bioactive compounds, cytotoxicity, ethanol, fluorouracil, free radical scavengers, glucosides, inhibitory concentration 50, lignans, neoplasm cells, nuclear magnetic resonance spectroscopy, roots, spectral analysis
Abstract:
In the search for biologically active compounds from the roots of Bupleurum chinense D C., phytochemical investigation of its ethanol extract led to the isolation and identification of a new 8- O -4′ neolignan glucoside, saikolignanoside A (1), along with eight known lignans (2–9). Their structures were determined on the basis of IR, UV, HRESIMS, and NMR spectroscopic analyses. The antioxidant and cytotoxic effects of isolated compounds were evaluated in vitro . The isolated compounds (IC ₅₀ > 200 μM) did not display 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Whereas compounds 1–2 , 5 , 7, and 9 exhibited potent 2, 2′-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging properties with IC ₅₀ values ranging from 8.34 to 15.24 μM, while compounds 3–4 , 6 , 8 showed moderate properties. In addition, all compounds were evaluated for cytotoxicities against A549, HepG2, U251, Bcap-37, and MCF-7 cell lines. Compounds 5 and 9 (IC ₅₀ < 51.62 μM) possessed stronger cytotoxic activities against all the tested tumor cell lines, compared with the positive control 5-Fluorouracil.
Agid:
5668416