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Light-induced protein degradation in human-derived cells

Sun, Wansheng, Zhang, Wenyao, Zhang, Chao, Mao, Miaowei, Zhao, Yuzheng, Chen, Xianjun, Yang, Yi
Biochemical and biophysical research communications 2017
Avena sativa, luciferase, oxygen, protein degradation
Controlling protein degradation can be a valuable tool for posttranslational regulation of protein abundance to study complex biological systems. In the present study, we designed a light-switchable degron consisting of a light oxygen voltage (LOV) domain of Avena sativa phototropin 1 (AsLOV2) and a C-terminal degron. Our results showed that the light-switchable degron could be used for rapid and specific induction of protein degradation in HEK293 cells by light in a proteasome-dependent manner. Further studies showed that the light-switchable degron could also be utilized to mediate the degradation of secreted Gaussia princeps luciferase (GLuc), demonstrating the adaptability of the light-switchable degron in different types of protein. We suggest that the light-switchable degron offers a robust tool to control protein levels and may serves as a new and significant method for gene- and cell-based therapies.