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Light-induced protein degradation in human-derived cells

Author:
Sun, Wansheng, Zhang, Wenyao, Zhang, Chao, Mao, Miaowei, Zhao, Yuzheng, Chen, Xianjun, Yang, Yi
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
Avena sativa, luciferase, oxygen, protein degradation
Abstract:
Controlling protein degradation can be a valuable tool for posttranslational regulation of protein abundance to study complex biological systems. In the present study, we designed a light-switchable degron consisting of a light oxygen voltage (LOV) domain of Avena sativa phototropin 1 (AsLOV2) and a C-terminal degron. Our results showed that the light-switchable degron could be used for rapid and specific induction of protein degradation in HEK293 cells by light in a proteasome-dependent manner. Further studies showed that the light-switchable degron could also be utilized to mediate the degradation of secreted Gaussia princeps luciferase (GLuc), demonstrating the adaptability of the light-switchable degron in different types of protein. We suggest that the light-switchable degron offers a robust tool to control protein levels and may serves as a new and significant method for gene- and cell-based therapies.