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Capillary electrophoresis coupled to mass spectrometry employing hexafluoro-2-propanol for the determination of nucleosides and nucleotide mono-, di- and tri-phosphates in baby foods

Mateos-Vivas, María, Domínguez-Álvarez, Javier, Rodríguez-Gonzalo, Encarnación, Carabias-Martínez, Rita
Food chemistry 2017 v.233 pp. 38-44
capillary electrophoresis, desserts, detection limit, fish, fruits, infant foods, mass spectrometry, meat, nucleosides, ultrafiltration
The present work describes a method for the simultaneous determination of unmodified nucleosides and nucleotide mono-, di- and tri-phosphates by capillary electrophoresis coupled to mass spectrometry (CE-MS). The use of hexafluoro-2-propanol (HFIP) in the separation medium, and as an additive to the sheath liquid of the electrospray interface (ESI), generated a highly efficient and sensitive method. Instrumental limits of detection in the range of 14–53ngmL−1 for nucleosides and 7–23, 20–49 and 64–124ngmL−1 for nucleotide mono-, di-, and tri-phosphates, respectively, were found. Sample treatment involved diluting an aliquot of baby food with ultra-high quality water and applying centrifugation-assisted ultrafiltration (CUF). The proposed method was validated and used to analyse a variety of baby food samples (16 in total) such as fish, meat, fruits, and baby dairy desserts that may endogenously contain these analytes.