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Gold nanoparticle-based low limit of detection Love wave biosensor for carcinoembryonic antigens

Li, Shuangming, Wan, Ying, Su, Yan, Fan, Chunhai, Bhethanabotla, Venkat R.
Biosensors & bioelectronics 2017 v.95 pp. 48-54
antibodies, antigens, biomarkers, biosensors, detection limit, electronics, immunoassays, nanogold, neoplasms, point-of-care systems, quartz
In this work, a Love wave biosensing platform is described for detecting cancer-related biomarker carcinoembryonic antigen (CEA). An ST 90°-X quartz Love wave device with a layer of SiO2 waveguide was combined with gold nanoparticles (Au NPs) to amplify the mass loading effect of the acoustic wave sensor to achieve a limit of detection of 37pg/mL. The strategy involves modifying the Au NPs with anti-CEA antibody conjugates to form nanoprobes in a sandwich immunoassay. The unamplified detection limit of the Love wave biosensor is 9.4ng/mL. This 2–3 order of magnitude reduction in the limit of detection brings the SAW platform into the range useful for clinical diagnosis. Measurement electronics and microfluidics are easily constructed for acoustic wave biosensors, such as the Love wave device described here, allowing for robust platforms for point of care applications for cancer biomarkers in general.