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Near- and mid-infrared spectroscopic determination of algal composition

Author:
Mulbry, Walter, Reeves, James, Liu, Yan, Ruan, Zhenhua, Liao, Wei
Source:
Journal of applied phycology 2012 pp. 1261
ISSN:
0921-8971
Subject:
accuracy, algae and seaweeds, calibration, equipment performance, fatty acid composition, least squares, lipid content, lipids, near-infrared reflectance spectroscopy, nitrogen content, phosphorus, scrubbers, species diversity, sugar content, sugars
Abstract:
The objective of this study was to evaluate whether near-infrared reflectance spectroscopy (NIRS) or mid-infrared reflectance spectroscopy (MIRS) could be used to determine the composition of algal turf scrubber samples. We assayed a set of algal turf scrubber (ATS) samples (n = 117) by NIRS, MIRS, and conventional means for ash, total sugar, mono-sugar, total N, and P content. A subset of these samples (n = 64) were assayed by conventional means, MIRS, and NIRS for total lipid and total fatty acid content. We developed calibrations using all the samples and a one-out cross-validation procedure under partial least-squares regression. This process was repeated using 75% of randomly selected samples to develop the calibration and the remaining samples as an independent test set. Results using the entire sample set demonstrated that NIRS and MIRS can accurately determine ash (r 2 = 0.994 and 0.995, respectively) and total N (r 2 = 0.787 and 0.820, respectively) content, but not phosphorus, total sugar, or mono-sugar content in ATS samples. Results using the 64 sample subset indicated that neither NIRS nor MIRS can accurately determine lipid or total fatty acid content in ATS samples.
Agid:
56731
Handle:
10113/56731