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Olegusella massiliensis gen. nov., sp. nov., strain KHD7T, a new bacterial genus isolated from the female genital tract of a patient with bacterial vaginosis

Diop, Khoudia, Diop, Awa, Bretelle, Florence, Cadoret, Frédéric, Michelle, Caroline, Richez, Magali, Cocallemen, Jean-François, Raoult, Didier, Fournier, Pierre-Edouard, Fenollar, Florence
Anaerobe 2017 v.44 pp. 87-95
Olsenella uli, anaerobes, bacteria, fatty acids, female genitalia, genes, new genus, patients, phenotype, phylogeny, ribosomal RNA, women
Strain KHD7ᵀ, a Gram-stain-positive rod-shaped, non-sporulating, strictly anaerobic bacterium, was isolated from the vaginal swab of a woman with bacterial vaginosis. We studied its phenotypic characteristics and sequenced its complete genome. The major fatty acids were C16:0 (44%), C18:2n6 (22%), and C18:1n9 (14%). The 1,806,744 bp long genome exhibited 49.24% G+C content; 1549 protein-coding and 51 RNA genes. Strain KHD7ᵀ exhibited a 93.5% 16S rRNA similarity with Olsenella uli, the phylogenetically closest species in the family Coriobacteriaceae. Therefore, strain KHD7ᵀ is sufficiently distinct to represent a new genus, for which we propose the name Olegusella massiliensis gen. nov., sp. nov. The type strain is KHD7ᵀ.