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Pectinase production by Aspergillus niger LB-02-SF is influenced by the culture medium composition and the addition of the enzyme inducer after biomass growth

Author:
Reginatto, Caroline, Rossi, Caroline, Miglioranza, Betina Gamba, Santos, Marielem dos, Meneghel, Lenara, Silveira, Mauricio Moura da, Malvessi, Eloane
Source:
Process biochemistry 2017 v.58 pp. 1-8
ISSN:
1359-5113
Subject:
Aspergillus niger, Citrus, biomass, culture media, developmental stages, glucose, microbial growth, mixing, oxygen, pH, pectins, polygalacturonase
Abstract:
The production of pectinase by Aspergillus niger LB-02-SF was focused on a submerged cultivation, before it was evaluated in a solid-state process. This study involved the creation of a defined culture medium and an evaluation of the effects of the addition of the enzyme inducer, citrus pectin, to the medium after the intense biomass growth phase. A culture medium formulated without glucose allowed a reduction of biomass growth and greater pectinase production, facilitated by the control of process parameters such as mixing, pH and oxygen supply. The addition of pectin when a minimum pH of 2.7 was reached at 22h of cultivation did not affect fungal growth. The maximum biomass concentration was 11.0g/L at 48h, a value similar to that observed for the control, in which pectin was included in the medium at the beginning of the process (11.5g/L, at 41h). However, this condition favored the production of 14U/mL pectinase, which was approximately 40% higher than the value observed for the control. These results show that pectinase production by A. niger in a submerged cultivation is strongly affected by the medium composition as well as the delayed addition of pectin to the fermentation broth.
Agid:
5673400