PubAg

Main content area

Irritable bowel syndrome: is it associated with genotypes of Blastocystis hominis

Author:
Yakoob, Javed, Jafri, Wasim, Beg, Mohammad Asim, Abbas, Zaigham, Naz, Shagufta, Islam, Muhammad, Khan, Rustam
Source:
Parasitology research 2010 v.106 no.5 pp. 1033-1038
ISSN:
0932-0113
Subject:
Blastocystis hominis, clinical examination, genetic variation, genotype, genotyping, hospitals, humans, microscopy, parasites, patients, polymerase chain reaction, universities
Abstract:
Blastocystis hominis is the most common intestinal parasite in humans. An extensive genetic variability has been described recently in B. hominis isolates. The aim of this study was to analyze genotypes of B. hominis isolates obtained from the healthy individuals and patients with irritable bowel syndrome-diarrhea (IBS-D). The patients with IBS-D were enrolled from gastroenterology outpatient department at the Aga Khan University Hospital. History and physical examination was done. Stool microscopy, culture, and polymerase chain reaction for B. hominis genotyping were carried out. The study included 158 patients with IBS-D, mean age 41 ± 15, age range 16–83 years, and male/female ratio of 109:49. One hundred fifty-seven (49.8%) were taken as healthy control. The dominant B. hominis genotypes were genotype 1 in 87 (65%) and type 3 in 49 (37%). In IBS-D, genotype 1 was present in 75 (86%; P < 0.001) compared to 12 (14%) in controls while type 3 was present in 23 (47%) compared to 26 (53%) in controls (P < 0.001), respectively. Infection with single genotype of B. hominis was present in 70 (73%) with IBS-D and in 26 (27%) in control group while with multiple genotypes in 25 (64%) in IBS-D and 14 (36%) in control group (P = 0.30), respectively. Majority of our patients had typeable B. hominis infection. The genotype of B. hominis in IBS-D was type 1 while in control genotype 3 was predominant.
Agid:
567418