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Immobilized lignin peroxidase from Ganoderma lucidum IBL-05 with improved dye decolorization and cytotoxicity reduction properties
- Shaheen, Raheela, Asgher, Muhammad, Hussain, Fatima, Bhatti, Haq Nawaz
- International journal of biological macromolecules 2017 v.103 pp. 57-64
- Artemia, Ganoderma lucidum, biocatalysts, biochemical oxygen demand, bioremediation, calcium alginate, calcium chloride, catalytic activity, chemical oxygen demand, cytotoxicity, decolorization, effluents, immobilized enzymes, industrial applications, lignin peroxidase, pH, reactive dyes, sodium alginate, temperature, textile industry, water quality
- Use of free microbial enzymes for bioremediation and other industrial applications has several disadvantages like low stability and non-reusability in repeated batch operations. Immobilized enzymes are stable, recoverable and reusable in industrial processes. In this scenario G. lucidum IBL-05 LiP was entrapped in Ca-alginate beads using optimum concentrations of Na-alginate (4%), calcium chloride (0.2M) and glutraldehyde (0.02%). Optimum pH (pH 5) and temperature (55°C) for entrapped LiP were improved as compared to free LiP. Catalytic behavior of LiP also significantly enhanced, as Km value (0.25mM) decreased and Vmax value (868.6μmol/min) increased after ca-alginate entrapment of LiP. Decolorization efficiencies of Sandal reactive dyes after treating with immobilized LiP were in the range of 80–93%. A significant reduction was observed in water quality parameters including, BOD (66.44–98.22%), COD (81.34–98.82%) and TOC (80.21–97.77%) values. The cytotoxicity values for heamolytic and brine shrimp lethality of dye solutions treated with Ca-alginate immobilized LiP reduced up to 2.10–5.06% and 5.43–9.23%, respectively. On the basis of reduced toxicity and cytotoxicity values, it was concluded that Ca-alginate beads entrapped LiP may be an effective biocatalyst for bioremediation of dye based textile industry effluents.