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Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates

Author:
Seto, Junji, Wada, Takayuki, Suzuki, Yu, Ikeda, Tatsuya, Mizuta, Katsumi, Mitarai, Satoshi, Ahiko, Tadayuki
Source:
Journal of microbiological methods 2017 v.139 pp. 12-14
ISSN:
0167-7012
Subject:
Mycobacterium tuberculosis, polymerase chain reaction, tandem repeat sequences, tuberculosis
Abstract:
Variable-number tandem-repeat typing for Mycobacterium tuberculosis clinical isolates contributes to evidence-based tuberculosis control. However, cumbersome PCR procedures for the typing have disturbed routine analyses. We proposed a convenient PCR method for the typing using a PCR master mix that provides rapidity and long-term stability of the frozen PCR cocktail.
Agid:
5676336