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Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates
- Seto, Junji, Wada, Takayuki, Suzuki, Yu, Ikeda, Tatsuya, Mizuta, Katsumi, Mitarai, Satoshi, Ahiko, Tadayuki
- Journal of microbiological methods 2017 v.139 pp. 12-14
- Mycobacterium tuberculosis, polymerase chain reaction, tandem repeat sequences, tuberculosis
- Variable-number tandem-repeat typing for Mycobacterium tuberculosis clinical isolates contributes to evidence-based tuberculosis control. However, cumbersome PCR procedures for the typing have disturbed routine analyses. We proposed a convenient PCR method for the typing using a PCR master mix that provides rapidity and long-term stability of the frozen PCR cocktail.