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Mesophilic anaerobic digestion of pig slurry and fruit and vegetable waste: Dissection of the microbial community structure

Author:
Ros, Margarita, de Souza Oliveira Filho, Jose, Perez Murcia, Maria Dolores, Bustamante, Maria Angeles, Moral, Raul, Coll, Maria Dolores, Lopez Santisima-Trinidad, Ana Belen, Pascual, Jose Antonio
Source:
Journal of cleaner production 2017 v.156 pp. 757-765
ISSN:
0959-6526
Subject:
Bacteroidetes, Firmicutes, Methanobrevibacter, Methanosaeta, anaerobic digestion, bacteria, biogas, community structure, methane, methane production, methanogens, microbial communities, pig manure, proteolysis, sludge, tomatoes
Abstract:
This paper studies the abiotic properties and the microbial community of a pilot scale digester with a working volume capacity of 300 L. The system worked at mesophilic operating conditions, with several organic load rates formed by a mixture of different ratios between waste A (fruit and vegetable sludge + tomato waste) and waste B (pig slurry). The core group of microorganisms corresponding to the ratio 70% A:30% B, showed the highest methane production (60%). This core group included bacteria, principally Firmicutes and Bacteroidetes, involved in cellulolytic degradation, proteolysis and acidogenesis, as well as a concomitance between acetotrophic, mainly Methanosaeta methanogens and hydrogenotrophic, principally Methanosphaera and Methanobrevibacter methanogens. An increase of the proportion of waste B (pig slurry) to 50–60% resulted in a decrease of the production of biogas and methane, which unbalanced the methanogenic bacteria, increasing the amount of principally hydrogenotropic methanogens (Methanosphaera and Methanobrevibacter).
Agid:
5677003