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New bioactive compounds from the marine-derived actinomycete Nocardiopsis lucentensis sp. ASMR2

Author:
Eliwa, Essam M., Abdel-Razek, Ahmed S., Frese, Marcel, Wibberg, Daniel, Halawa, Ahmed H., El-Agrody, Ahmed M., Bedair, Ahmed H., Kalinowski, Jörn, Sewald, Norbert, Shaaban, Mohamed
Source:
Zeitschrift für Naturforschung 2017 v.72 no.5 pp. 351-360
ISSN:
1865-7117
Subject:
Nocardiopsis lucentensis, adenosine, antimicrobial properties, bioactive compounds, carboxylic acids, cervix, chromatography, coasts, cytotoxicity, fermentation, glycerol, human cell lines, humans, indole acetic acid, ionization, mass spectrometry, microorganisms, multiple drug resistance, neoplasm cells, nuclear magnetic resonance spectroscopy, rice, Red Sea
Abstract:
In the search for new bioactive compounds from extremophilic actinomycetes, a new marine actinomycete strain, Nocardiopsis lucentensis sp. ASMR2 has been isolated and taxonomically identified from marine plants collected in the Red Sea at Hurghada coasts. A large-scale fermentation of the strain on modified rice solid medium was performed, followed by work-up and purification of the obtained extract using a series of chromatographic purifications, delivering the novel butenolide system 3′-hydroxy-N-(2-oxo-2,5-dihydrofuran-4-yl)propionamide (1a) along with the naturally new 4-methoxy-2H-isoquinolin-1-one (2). Furthermore, eight known bioactive compounds are also reported, namely, indole-3-carboxylic acid, indole-3-acetic acid, indole-3-acetic acid methyl ester, furan-2,5-dimethanol, tyrosol, glycerol linoleate, cyclo-(Tyr, Pro), and adenosine. The chemical structures of the new compounds (1a, 2) were confirmed by extensive one- and two-dimensional (1D and 2D) nuclear magnetic resonance (NMR) spectroscopy, electron ionization high resolution (EI-HR) mass spectrometry, and by comparison with literature data. The antimicrobial activity of the strain extract, as well as of compounds 1a and 2, were studied using a panel of pathogenic microorganisms. The in vitro cytotoxicity of the bacterial extract and compounds 1a and 2 were studied against the human cervix carcinoma cell line (KB-3-1) and its multidrug-resistant subclone (KB-V1).
Agid:
5678670