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Optimization of SNAP-25-derived peptide substrate for improved detection of botulinum A in the Endopep-MS assay
- Rosen, Osnat, Feldberg, Liron, Dor, Eyal, Gura, Sigalit, Zichel, Ran
- Analytical biochemistry 2017 v.528 pp. 34-37
- botulinum toxin, proteins, toxicity
- Botulinum neurotoxins (BoNTs) are the most toxic proteins in nature. Endopeptidase-mass-spectrometry (Endopep-MS) is used as a specific and rapid in-vitro assay to detect BoNTs. In this assay, immunocaptured toxin cleaves a serotype-specific-peptide-substrate, and the cleavage products are then detected by MS. Here we describe the design of a new peptide substrate for improved detection of BoNT type A (BoNT/A). Our strategy was based on reported BoNT/A-SNAP-25 interactions integrated with analysis method efficiency considerations. Integration of the newly designed substrate led to a 10-fold increase in the assay sensitivity both in buffer and in clinically relevant samples.