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Mutation of Spirulina sp. by nuclear irradiation to improve growth rate under 15% carbon dioxide in flue gas

Cheng, Jun, Lu, Hongxiang, He, Xin, Yang, Weijuan, Zhou, Junhu, Cen, Kefa
Bioresource technology 2017 v.238 pp. 650-656
Spirulina, biomass production, carbon dioxide, carbon dioxide enrichment, carbon dioxide fixation, cell walls, cobalt, domestication, flue gas, fractal dimensions, gamma radiation, irradiation, mutants, mutation, phenotype, porosity, power plants, radionuclides, ultrastructure
Spirulina sp. was mutated by γ-rays from 60Co nuclear irradiation to improve growth and CO2 fixation rate under 15vol.% CO2 (in flue gas from a power plant). Mutants with enhanced growth phenotype were obtained, with the best strain exhibiting 310% increment in biomass yield on day 4. The mutant was then domesticated with elevated CO2 concentration, and the biomass yield increased by 500% after domestication under 15vol.% CO2, with stable inheritance. Ultrastructure of Spirulina sp. shows that the fractal dimension of Spirulina cells decreased by 23% after mutation. Pore size in the cell wall of Spirulina mutant increased by 33% after 15vol.% CO2 domestication. This characteristic facilitated the direct penetration of CO2 into cells, thus improving CO2 biofixation rate.