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Mutation of Spirulina sp. by nuclear irradiation to improve growth rate under 15% carbon dioxide in flue gas

Author:
Cheng, Jun, Lu, Hongxiang, He, Xin, Yang, Weijuan, Zhou, Junhu, Cen, Kefa
Source:
Bioresource technology 2017 v.238 pp. 650-656
ISSN:
0960-8524
Subject:
Spirulina, biomass production, carbon dioxide, carbon dioxide enrichment, carbon dioxide fixation, cell walls, cobalt, domestication, flue gas, fractal dimensions, gamma radiation, irradiation, mutants, mutation, phenotype, porosity, power plants, radionuclides, ultrastructure
Abstract:
Spirulina sp. was mutated by γ-rays from 60Co nuclear irradiation to improve growth and CO2 fixation rate under 15vol.% CO2 (in flue gas from a power plant). Mutants with enhanced growth phenotype were obtained, with the best strain exhibiting 310% increment in biomass yield on day 4. The mutant was then domesticated with elevated CO2 concentration, and the biomass yield increased by 500% after domestication under 15vol.% CO2, with stable inheritance. Ultrastructure of Spirulina sp. shows that the fractal dimension of Spirulina cells decreased by 23% after mutation. Pore size in the cell wall of Spirulina mutant increased by 33% after 15vol.% CO2 domestication. This characteristic facilitated the direct penetration of CO2 into cells, thus improving CO2 biofixation rate.
Agid:
5681265