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DNA Methylation Down-Regulates EGFR Expression in Chickens

Author:
Luo, Juan, Chang, Shuang, Zhang, Huanmin, Li, Bichun, Song, Jiuzhou
Source:
Avian diseases 2013 v.57 no.2 pp. 366
ISSN:
0005-2086
Subject:
DNA methylation, Mardivirus, RNA-directed DNA polymerase, chickens, enzyme activation, epidermal growth factor, gene expression regulation, growth promotion, neoplasms, receptors, therapeutics, tyrosine, viruses
Abstract:
The epidermal growth factor receptor (EGFR), a growth-factor-receptor tyrosine kinase, is up-regulated in numerous tumors, which provides a good target for cancer therapy. Although it has been documented that oncoviruses are responsible for the activation of EGFR in tumors, the impact of Marek's disease virus (MDV) infection on EGFR has not yet been studied. We performed quantitative reverse transcriptase (RT)-PCR to check EGFR expression and found that it was significantly down-regulated after MDV infection. To explore the mechanism of EGFR repression, we examined the level of methylation of the EGFR promoter. The methylation level was significantly increased at 21 days postinfection, indicating a potential role of promoter methylation in EGFR repression.
Agid:
56854
Handle:
10113/56854