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Assessment of genetic diversity on snake melon (Cucumis melo var. flexuosus) using ISSR markers in Iran
- Dastranji, N., Shojaeiyan, A., Falahati-Anbaran, M.
- Acta horticulturae 2017 no.1151 pp. 45-50
- Cucumis melo subsp. melo var. flexuosus, cluster analysis, drought, genes, genetic distance, genetic variation, microsatellite repeats, pathogens, pests, salinity, Iran
- Local accessions have obtained traits that improved their adaptation to local conditions. These accessions may contain genes responsible for resistance to drought, salinity and pathogens and pests. Iran is assumed to be part of diversity centers of Cucumis melo var. flexuosus. Because of high economic importance of the plant and little attention paid to it, it is necessary to invest more efforts in genetic studies on local accessions. The genetic diversity among 16 accessions was assessed by variation at Inter-Simple Sequence Repeat marker bands using 10 primers. A total of 93 bands were identified with an average of 8.2 polymorphic bands per primer. The average level of polymorphism was 82.95%. The average of polymorphic information contents (PIC) and marker index was 0.34 and 2.58, respectively. Genetic diversity within populations was 85% while it was 15% among populations. Cluster analysis was carried out by UPGMA method and Nei's genetic distance coefficient. Cluster analysis classified the accessions into 5 major groups.