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Excretory/secretory products from the gastrointestinal nematode Trichuris muris

Author:
Tritten, Lucienne, Tam, Mifong, Vargas, Mireille, Jardim, Armando, Stevenson, Mary M., Keiser, Jennifer, Geary, Timothy G.
Source:
Experimental parasitology 2017
ISSN:
0014-4894
Subject:
Trichuris muris, chemical elements, excretory-secretory products, gastrointestinal nematodes, immune system, mass spectrometry, mice, microRNA, nematode infections, protein composition
Abstract:
To better control gastrointestinal nematode infections in humans and animals, it is important to understand the strategies used by these parasites to modulate the host immune system. In this regard, molecules released by parasites have been attributed crucially important roles in host-parasite negotiations. We characterized the excretory/secretory (E/S) microRNA (miRNA) and protein profiles from the mouse gastrointestinal nematode parasite Trichuris muris. Released miRNAs were subjected to miRNA sequencing and E/S proteins were analysed by mass spectrometry. Fourteen miRNAs were identified in T. muris exosome-like vesicles, as well as 73 proteins of nematode origin, 11 of which were unique to this study. Comparison with published nematode protein secretomes revealed high conservation at the functional level.
Agid:
5690033