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CRISPR/dCas9-mediated inhibition of gene expression in Staphylococcus aureus
- Dong, Xiaoyun, Jin, Yingli, Ming, Di, Li, Bangbang, Dong, Haisi, Wang, Lin, Wang, Tiedong, Wang, Dacheng
- Journal of microbiological methods 2017 v.139 pp. 79-86
- Staphylococcus aureus, gene expression regulation, genes, genetic engineering
- The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method.