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EHS matrix incubated in media containing penicillin retains sufficient concentrations of antibiotic to inhibit growth of susceptible microorganisms

Author:
Murphy, Mark P., Niedziela, Dagmara A., Keane, Orla M.
Source:
Journal of microbiological methods 2017 v.139 pp. 103-106
ISSN:
0167-7012
Subject:
Staphylococcus aureus, bacteria, cell culture, penicillins
Abstract:
In studying the interaction between bacteria and host cells in vitro, the latter are frequently cultured on commercially available biotic matrices such as Matrigel® or Geltrex®. To avoid contamination, host cells are commonly grown in the presence of antibiotics. However, we present here the finding that cell culture on such a matrix in the presence of antibiotics interferes with the outcome of subsequent infection experiments by virtue of diminished bacterial survival. By comparing outcomes for penicillin-susceptible and resistant strains of Staphylococcus aureus, we show that residual penicillin remains in the matrix despite the antibiotics' withdrawal from culture. Hence, the use of antibiotics should be avoided in this context.
Agid:
5690835