Main content area

Mono- and co-substrate utilization kinetics using mono- and co-culture of Clostridium beijerinckii and Clostridium saccharoperbutylacetonicum

Nasr, Noha, Gupta, Medhavi, Hafez, Hisham, El Naggar, M. Hesham, Nakhla, George
Bioresource technology 2017
Clostridium beijerinckii, Clostridium saccharoperbutylacetonicum, acetates, butyrates, cellulose, coculture, fermentation, glucose, hydrogen, hydrogen production, propionic acid, starch
The effect of co-culturing C. beijerinckii and C. saccharoperbutylacetonicum for H2 production using mono- and co-substrates of glucose, starch, and cellulose was assessed. Monod kinetic parameters (K, maximum specific substrate utilization rate; and Ks, half-saturation constant) of the C. beijerinckii, C. saccharoperbutylacetonicum, and the co-culture were determined. Co-cultures utilizing glucose competed for the substrate, but showed enhancement for utilizing starch. The maximum values for K on glucose and starch were 0.48g substrate/gVSS.h achieved by C. saccharoperbutylacetonicum mono-culture and 0.39g substrate/gVSS.h achieved by the co-culture, respectively. The average Ks for all mono- and co-culture experiments was 0.93±0.03g/L. Acetate, butyrate, and propionate were the main fermentation products for all experiments. Maximum H2 production yields on glucose (2.69mol/molglucose) and starch (1.07mol/molhexose) were achieved by C. beijerinckii and C. saccharoperbutylacetonicum mono-cultures, respectively; however, neither culture was able to degrade cellulose as a mono-substrate.