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Comparative Proteomic Analysis Reveals the Effects of Exogenous Calcium against Acid Rain Stress in Liquidambar formosana Hance Leaves

Hu Wen-Jun, Wu Qian, Liu Xiang, Shen Zhi-Jun, Chen Juan, Liu Ting-Wu, Zhu Chun-Quan, Wu Fei-Hua, Chen Lin, Wei Jia, Qiu Xiao-Yun, Shen Guo-Xin, Zheng Hai-Lei
Journal of Proteome Research 2016 v.15 no.1 pp. 216-228
Calvin cycle, Liquidambar formosana, Western blotting, acid deposition, antioxidant activity, calcium, chlorophyll, energy, forest health, forest trees, genes, leaching, leaves, protective effect, protein synthesis, proteins, proteome, proteomics, quantitative polymerase chain reaction, reactive oxygen species, reverse transcriptase polymerase chain reaction, soil, translation (genetics), woody plants
Acid rain (AR) impacts forest health by leaching calcium (Ca) away from soils and plants. Ca is an essential element and participates in various plant physiological responses. In the present study, the protective role of exogenous Ca in alleviating AR stress in Liquidambar formosana Hance at the physiological and proteomic levels was examined. Our results showed that low Ca condition resulted in the chlorophyll content and photosynthesis decreasing significantly in L. formosana leaves; however, these effects could be reversed by high Ca supplementation. Further proteomic analyses successfully identified 81 differentially expressed proteins in AR-treated L. formosana under different Ca levels. In particular, some of the proteins are involved in primary metabolism, photosynthesis, energy production, antioxidant defense, transcription, and translation. Moreover, quantitative real time polymerase chain reaction (qRT-PCR) results indicated that low Ca significantly increased the expression level of the investigated Ca-related genes, which can be reversed by high Ca supplementation under AR stress. Further, Western blotting analysis revealed that exogenous Ca supply reduced AR damage by elevating the expression of proteins involved in the Calvin cycle, reactive oxygen species (ROS) scavenging system. These findings allowed us to better understand how woody plants respond to AR stress at various Ca levels and the protective role of exogenous Ca against AR stress in forest tree species.