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Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.)

Author:
Wang, He, Fan, Mingxia, Wang, Guohong, Zhang, Chunyu, Shi, Lei, Wei, Zhengyi, Ma, Wenjuan, Chang, Jing, Huang, Senxin, Lin, Feng
Source:
Genome 2017 v.60 no.6 pp. 485-495
ISSN:
1480-3321
Subject:
Zea mays, chromosomes, corn, gene fusion, genes, genetically modified plants, pollen, pollen tubes, promoter regions, protein-serine-threonine kinases, sequence analysis, serine, start codon, threonine, tissues
Abstract:
ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.
Agid:
5694935