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Genetic analysis of net form net blotch resistance in barley lines CIho 5791 and Tifang against a global collection of P. teres f. teres isolates

V. M. Koladia, J. D. Faris, J. K. Richards, R. S. Brueggeman, S. Chao, T. L. Friesen
Theoretical and applied genetics 2017 v.130 no.1 pp. 163-173
Pyrenophora teres, barley, chromosome mapping, chromosomes, disease resistance, genetic analysis, genome, inbred lines, net blotch, plant breeding, quantitative trait loci, single nucleotide polymorphism
The barley line CI5791 confers high levels of resistance to Pyrenophora teres f. teres, causal agent of net form net blotch (NFNB), with few documented isolates overcoming this resistance. Tifang barley also harbors resistance to P. teres f. teres which was previously shown to localize to barley chromosome 3H. A CI5791 × Tifang F(6) recombinant inbred line (RIL) population was developed using single seed descent. The Illumina iSelect SNP platform was used to identify 2,562 single nucleotide polymorphism (SNP) markers across the barley genome, resulting in seven linkage maps, one for each barley chromosome. The CI5791 × Tifang RIL population was evaluated for NFNB resistance using nine P. teres f. teres isolates collected globally. Tifang was resistant to four of the isolates tested whereas CI5791 was highly resistant to all nine isolates. QTL analysis indicated that the CI5791 resistance mapped to chromosome 6H whereas the Tifang resistance mapped to chromosome 3H. Additionally, CI5791 also harbored resistance to two Japanese isolates that mapped to a 3H region similar to that of Tifang. SNP markers and RILs harboring both 3H and 6H resistance will be useful in resistance breeding against NFNB.