U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Determination of citrinin using molecularly imprinted solid phase extraction purification, HPLC separation, and fluorescence detection

Michael Appell, Michael A. Jackson, Lijuan C. Wang, Wayne B. Bosma
Journal of Liquid Chromatographuy & Related Technologies 2015 v.38 no.20 pp. 1815-1819
citrinin, corn, fluorescence, high performance liquid chromatography, liquids, molecular imprinting, polymers, purification methods, separation, solid phase extraction, sorption isotherms, synthesis
A liquid chromatography based method to detect citrinin in corn was developed using molecularly imprinted solid phase extraction (MISPE) sample clean-up. Molecularly imprinted polymers were synthesized using 1,4-dihydroxy-2-naphthoic acid as the template and an amine functional monomer. Density functional calculations suggest the mimic template interacts with the functional monomer in a similar manner as citrinin. Freundlich isotherm analysis indicated the template provided a significant imprinting effect for citrinin binding. A high performance liquid chromatography with fluorescence detection (HPLC-FD) method to detect citrinin in maize was developed utilizing the imprinted polymers for sample clean-up (excitation at 330 nm; emission at 500 nm). Recoveries of citrinin in spiked corn samples (0.03-3 µg g(-1)) were between 82.3-91.5%. This study demonstrates that molecularly imprinted polymers are applicable in the solid phase extraction clean-up of corn samples for citrinin determination by HPLC-FD.