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In Vitro Analysis of the Immunomodulating Effects of Allium Hookeri on Lymphocytes,Macrophages, and Tumour Cells

Lee, Youngsub, Hyen Lee, Sung, Sun Jeong, Mi, Kim, Jung Bong, Hee Jang, Hwan, Choe, Jeong-sook., Kim, Dong Wook, Lillehoj, Hyun S.
The Journal of Poultry Science 2017 v.54 no.2 pp. 142-148
2,2-diphenyl-1-picrylhydrazyl, Allium hookeri, antioxidant activity, cell viability, chickens, ethanol, free radical scavengers, immunomodulators, in vitro culture, in vitro studies, innate immunity, leaves, lymphocyte proliferation, lymphocytes, macrophages, neoplasm cells, nitric oxide, plant extracts, roots, spleen
The present study investigated the effects of ethanol extracts of Allium hookeri (leaf, root, and fermented root) on parameters of innate immunity, tumour cell viability and antioxidant effect in vitro. Innate immunity was measured by spleen lymphocyte proliferation, nitric oxide production by chicken macrophage HD11 cells and suppressive effect on tumour cell viability was assessed using chicken RP9 cells. Free radical scavenging capacity as a measure of antioxidant capacity was determined by 0.15mM of DPPH solution. In vitro culture of chicken spleen lymphocytes with ethanol extract of Allium hookeri (62.5-500 µg/mL) significantly induced higher proliferation compared with media control. Stimulation of macrophages with ethanol extract of Allium hookeri (62.5-500 µg/mL) showed increased Nitric oxide production. Tumor cells growth was significantly inhibited by extracts of Allium hookeri at 15.6–125 µg/mL compared with medium control and all extracts exhibited greater than 80% scavenging activity at 1000 µg/mL compared with ethanol vehicle control. Above all, fermented root extracts showed strongest effects on antioxidant activity compared to leaf and root extracts.