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Abscisic Acid and Its Catabolites in Leaves and Berries of Chardonnay are Affected by Water Status

Author:
Balint, Gabriel, Reynolds, Andrew G.
Source:
Journal of plant growth regulation 2016 v.35 no.4 pp. 1126-1140
ISSN:
0721-7595
Subject:
Vitis vinifera, abscisic acid, biochemical pathways, evapotranspiration, fruit set, glucose, grapes, high performance liquid chromatography, irrigation management, leaves, mass spectrometry, metabolites, oxidation, phaseic acid, small fruits, vines, vineyards, water stress, wine cultivars, Ontario
Abstract:
The biosynthetic pathway of abscisic acid (ABA) is well known. The aim of this study was to investigate the relationship among various ABA catabolites in leaves and berries of Chardonnay grapevines grown under various irrigation regimes. An irrigation trial was set up in one vineyard, located in Niagara-on-the-Lake, ON, Canada, consisting of seven treatments: control (non-irrigated), plus three water levels (100, 50, and 25 % of estimated crop evapotranspiration) combined with two irrigation imposition times (fruit set, veraison). No irrigation occurred prior to treatment imposition. ABA, phaseic acid (PA), dihydrophaseic acid (DPA), 7′-hydroxy-ABA, 8′-hydroxy-ABA, neophaseic acid, and ABA glucose ester (ABA-GE) were quantified in leaves and berries by HPLC–MS. ABA was likely catabolized by conjugation to form ABA-GE in treatments under high levels of water deficit, while in treatments with high water status, the oxidation pathway leading to DPA or PA predominated. Concentrations of ABA and its catabolites therefore reflected vine water status, whereby the specific ABA catabolic pathways in leaves and berries were determined by water status level. Hormonal profiles suggested a direct relationship between ABA and vine water status. The concentration of ABA in Chardonnay may explain why and how white cultivars adapt to drought stress versus red cultivars.
Agid:
5696925