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Depollution potential of three macrophytes: Exudated, wall-bound and intracellular peroxidase activities plus intracellular phenol concentrations

Larue, Camille, Korboulewsky, Nathalie, Wang, Runying, Mévy, Jean-Philippe
Bioresource technology 2010 v.101 no.20 pp. 7951-7957
Iris pseudacorus, Phragmites australis, Typha latifolia, cell walls, constructed wetlands, enzyme activity, food industry, gallic acid, heavy metals, macrophytes, organic wastes, peroxidase, phenol, proteins, roots, sewage sludge, wastewater treatment
The aim of this study was to investigate the potential role of three macrophyte species (Iris pseudacorus, Typha latifolia and Phragmites australis) for detoxication of xenobiotics, and to study their variations with seasons or concentrations of sewage sludge from the food industry. For this purpose, some aspects of the green liver concept were explored through peroxidase measurements in three compartments in roots: intracellular, cell wall and extracellular. In addition, phenol concentrations were also measured in order to assess heavy metal detoxication potential. Enzyme activities and phenol concentrations were overall lower in winter according to the phenological stages and some sludge effects occurred. Results show that P. australis roots exuded and contained more peroxidase in all seasons: 17U/g (1373U/g protein), 0.8U/g (613U/g protein) and 4.8U/g (1329U/g protein) in intracellular compartments, cell wall and exudates, respectively. In contrast, the highest phenol concentration was found in I. pseudacorus roots: 143.3mgeq. gallic acid/g. Hence, in constructed wetlands, P. australis is suitable for organic waste water treatment, while I. pseudacorus should be used in the case of waters highly charged with heavy metals.