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Enediol-Ligands-Encapsulated Liposomes Enables Sensitive Immunoassay: A Proof-of-Concept for General Liposomes-Based Photoelectrochemical Bioanalysis

Mei, Li-Ping, Liu, Fei, Pan, Jian-Bin, Zhao, Wei-Wei, Xu, Jing-Juan, Chen, Hong-Yuan
Analytical chemistry 2017 v.89 no.12 pp. 6300-6304
antibodies, electrodes, encapsulation, immunoassays, nanoparticles, titanium dioxide
Sensitive photoelectrochemical (PEC) bioanalysis usually relies on enzyme-assisted signal amplification. This work describes the first proof-of-concept study for liposome-based PEC bioanalysis. Specifically, unilamellar liposomes were prepared and then utilized to carry the enediol-ligands and antibodies within their internal cavities and upon their external surfaces, respectively. On the other hand, the 96-well plate was used for accommodating the sandwich immunocomplexing, and then the confined liposomes were directed to release the encapsulated enediol-ligands into an individual well. The subsequent in situ sensitization of the TiO₂ nanoparticles (NPs) electrode was then used to transduce the recognition events. This facile strategy allows for sensitive immunoassay without the involvement of laborious electrode fabrication and enzymatic amplification. Importantly, the protocol can be extended as a general PEC method for numerous other targets of interest. We believe this work could offer a new perspective for the rational implementation of various liposome complexes for novel PEC bioanalysis.