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Characterization of Copy Number Variation’s Potential Role in Marek's Disease

Xu, Lingyang, He, Yanghua, Ding, Yi, Sun, Guirong, Carrillo, Jose Adrian, Li, Yaokun, Ghaly, Mona M., Ma, Li, Zhang, Huanmin, Liu, George E., Song, Jiuzhou
International Journal of Molecular Sciences 2017 v.18 no.5 pp. 1020-1034
Marek disease, chickens, disease resistance, gene deletion, gene dosage, gene expression, genetic resistance, genotyping, models, quantitative polymerase chain reaction, sequence analysis, single nucleotide polymorphism, strains
Marek's Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72 as well as their recombinant congenic strains (RCS) with varied susceptibility to MD are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we investigated copy number variations (CNV) in these inbred chicken lines using the 600 K Affymetrix Axiom HD SNP genotyping array. We detected 393 CNV segments across all ten chicken lines, of which 12 CNVs were specifically identified in Line 72. We then assessed genetic structure based on CNV and observed markedly different patterns. Finally, we validated two deletion events in Line 72 and correlated them with genes expression using qPCR and RNA-seq, respectively. Our combined results indicated that these two CNV deletions were likely to contribute to MD resistance/susceptibility.