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Temporal changes of microRNA gga-let-7b and gga-let-7i expression in chickens challenged with subgroup J avian leukosis virus

Author:
Ji, Jun, Shang, Huiqin, Zhang, Huanmin, Li, Hongxin, Ma, Jingyun, Bi, Yingzuo, Xie, Qingmei
Source:
Veterinary research communications 2017 v.41 no.3 pp. 219-226
ISSN:
0165-7380
Subject:
Avian leukosis virus, Internet, antineoplastic activity, bone marrow, carcinogenesis, cell cycle, chickens, computer software, gene expression, hemangioma, liver, microRNA, mitogen-activated protein kinase, myeloma, signal transduction, specific pathogen-free animals, strains, temporal variation, transforming growth factor beta, tumor suppressor protein p53
Abstract:
Two important microRNAs, gga-let-7b and gga-let-7i were examined for the relative expression in liver and bone marrow tissues from specific pathogen free chickens that were challenged either with GD1109 or NX0101 strain of subgroup J avian leukosis virus (ALV-J). The GD1109 strain of ALV-J reportedly causes hemangioma (HE) and NX0101 reportedly causes myeloma (ML) in susceptible chickens. Temporal changes of both gga-let-7b and gga-let-7i expression in ALV-J infected chickens were observed in contrast to its counterpart of a non-infected negative control group of chickens (P < 0.05 or P < 0.01) during the first 120 days post infection. Use of the web-based computational DIANA-mirPath software (available at http://microrna.gr/mirpath), it was predicted that both gga-let-7b and gga-let-7i were involved in multiple pathways including signaling pathways, such as MAPK, TGF-beta, Notch, Wnt, mTOR, Cell cycle, P53 and Jak-STAT. Combining our experimental data with reports on the microRNAs, we suggest that both gga-let-7i and gga-let-7b may also act as tumor suppressors in chicken, especially play a critical role in tumorigenesis induced by ALV-J.
Agid:
5700720
Handle:
10113/5700720