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Prevalence of Escherichia coli O157:H7 in beef cattle at slaughter and beef carcasses at retail shops in Ethiopia

Abdissa, Rosa, Haile, Woynshet, Fite, Akafete Teklu, Beyi, Ashenafi Feyisa, Agga, Getahun E., Edao, Bedaso Mammo, Tadesse, Fanos, Korsa, Mesula Geloye, Beyene, Takele, Beyene, Tariku Jibat, De Zutter, Lieven, Cox, Eric, Goddeeris, Bruno Maria
BMC Infectious Diseases 2017 v.17 no.1 pp. 277-283
Escherichia coli O157, amoxicillin, antibiotic resistance, beef carcasses, beef cattle, butcher shops, cefoxitin, cutting boards, developing countries, epidemiology, feces, food contact surfaces, hands, humans, infectious diseases, meat processing plants, microbial detection, microbiological risk assessment, slaughter, storage conditions, Ethiopia
Background: There is paucity of information regarding the epidemiology of Escherichia coli O157: H7 in developing countries. In this study, we investigated the occurrence of E. coli O157: H7 associated with beef cattle at processing plants and at retail shops in Ethiopia. Methods: Various samples were collected from beef cattle at slaughter/processing plants, carcass at retail shops and humans at health centers. E. coli O157: H7 was isolated, identified and characterized for antimicrobial resistance, using standard microbiological methods. Results: At the processing plants E. coli O157: H7 was detected in 1.89% of fecal, 0.81% of intestinal mucosal swab, 0.54% of skin swab and 0.54% of carcass internal swab samples. At retail shops it was detected in 0.8% of carcass and 0.8% of cutting board swab samples, while all samples from utensils, hands from workers, and fecal and stool samples were negative. All isolates were resistant to Amoxicillin, moderately resistant to Cefoxitine and Nitrofurantoins but susceptible to other antimicrobials tested. Conclusions: E. coli O157: H7 occurs at low prevalence in beef cattle, and the current sanitary dressing procedures in the processing plants and storage conditions in the retail shops are effective against E. coli O157: H7.