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Antimicrobial-Resistant Fecal Bacteria from Ceftiofur-Treated and Nonantimicrobial-Treated Comingled Beef Cows at a Cow-Calf Operation

Getahun E. Agga, John W. Schmidt, Terrance M. Arthur
Microbial Drug Resistance 2016 v.22 no.7 pp. 598-608
acid tolerance, cow-calf operations, sulfamethoxazole, tetracycline, animal husbandry, antibiotic resistance, beef cows, ceftiofur, bacteria, multiple drug resistance, nalidixic acid, trimethoprim, Enterococcus, feces, erythromycin, Salmonella enterica, Escherichia coli
We compared the occurrences of 3rd-generation cephalosporin-resistant (3GCr), tetracycline-resistant (TETr), and trimethoprim-sulfamethoxazole-resistant (COTr) Escherichia coli, 3GCr and nalidixic acid-resistant (NALr) Salmonella enterica, and erythromycin-resistant (ERYr) enterococci from the fecal samples of ceftiofur-treated (n = 162) and nonantimicrobial-treated (n = 207) comingled beef cows ≥8 years old, for which complete antimicrobial treatment records were available. The prevalence of 3GCr (17%; n = 369), TETr (88%), COTr E. coli (22%), and ERYr enterococci (69%) was not significantly (p > 0.05) associated with ceftiofur treatment, prior history of other antimicrobial treatments, or duration of time between last antimicrobial treatment and sampling. 3GCr and NALr S. enterica were not detected. The prevalence of tetB was significantly (p < 0.05) higher compared with tetA among TETr E. coli. However, the prevalence of tetA was significantly (p < 0.05) higher than tetB among 3GCr and COTr E. coli. There was a significant (p < 0.05) association between tetM and ermB among ERYr enterococci. In conclusion, occurrences of 3GCr, TETr, and COTr E. coli and ERYr enterococci in comingled antimicrobial-treated and nonantimicrobial-treated beef cows were not associated with ceftiofur or other antimicrobial use, indicating that other factors influenced the observed levels of antimicrobial-resistant bacteria in feces of beef cows.