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Discrimination of geographical origin of Napirira bean (Phaseolus vulgaris L.) based on phenolic profiles and antioxidant activity

Fan, Gongjian, Beta, Trust
Subtropical plant science 2017 v.62 pp. 217-222
2,2-diphenyl-1-picrylhydrazyl, 4-hydroxybenzoic acid, Phaseolus vulgaris, antioxidant activity, beans, catechin, farms, ferulic acid, free radical scavengers, p-coumaric acid, principal component analysis, protocatechuic acid, provenance, Malawi
The aims of the current work were to study the influence of geographical areas on phenolic compounds and antioxidant activity in Napirira beans (Phaseolus vulgaris L.) from different bean-growing areas of Malawi, and to evaluate the possibility of establishing a classification based on the geographical areas of the bean-growing regions. The statistical analysis showed significant differences (p<0.05) in free and bound phenolic content (0.67–1.70mg GAE/g and 0.36–0.75mg GAE/g) as well as the DPPH free radical scavenging capacity (0.51–1.48mg TE/g and 0.37–0.67mg TE/g) and ORAC assay (6.20–12.86μmol TE/g and 2.24–4.47μmol TE/g) between some of the different sampled farms, showing a significant effect of the bean-producing region on these parameters. Phenolic compounds including protocatechuic acid, p-hydroxybenzoic acid, catechin, p-coumaric acid, ferulic acid and sinapinic acid were identified and quantified in free phenolic extracts. Results of principal component analysis (PCA) and hierarchical cluster analysis (HCA) indicated that total phenolic content, antioxidant activity and content of individual phenolic acids could serve as parameters to establish a Napirira bean classification according to the geographical area of the bean-growing regions.