Main content area

The novel contributors of anti-diabetic potential in mulberry polyphenols revealed by UHPLC-HR-ESI-TOF-MS/MS

Li, Fuhua, Zhang, Bin, Chen, Gu, Fu, Xiong
Food research international 2017 v.100 pp. 873-884
Morus alba, alpha-glucosidase, antioxidant activity, correlation, delphinidin, ethanol, islets of Langerhans, keracyanin, mulberries, polyphenols, quercetin, rutin, syringic acid, taxifolin
To explore the comprehensive phenolic profile of mulberry (Morus alba L.) fruit and reveal the novel anti-diabetic constituents, mulberry polyphenols (MP) were fractionated through gradient ethanol elution and subjected to composition and bioactivity analysis. Forty-seven phenolic compounds were identified and quantified through UHPLC-HR-ESI-TOF-MS/MS, including twenty-one newly identified compounds such as delphinidin 3-rutinoside-5-glucoside and cyanidin 3-rutinoside dimer. The antioxidant activity and anti-diabetic potential of fractionated MP were analyzed in vitro and ex vivo with HepG2 and pancreatic β-cell RIN-m5F. Hierarchical cluster analysis and Pearson correlation coefficients revealed compounds such as syringic acid and galloylcyanidin-glycoside contributed most to inhibit α-glucosidase activity. Quercetin and cyanidin-glycosides were essential for cellular antioxidant activity. Dihydroquercetin and 1,5-dicaffeoylquinic acid were newly identified and validated as the main contributors after rutin to protect RIN-m5F cells against glucotoxicity. Such findings suggest the promising role of MP for type II diabetics and lay the foundation of further utilization and investigation.