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A high fat diet enhances the sensitivity of chick adipose tissue to the effects of centrally injected neuropeptide Y on gene expression of adipogenesis-associated factors

Author:
Wang, Guoqing, Williams, Carli A., McConn, Betty R., Cline, Mark A., Gilbert, Elizabeth R.
Source:
Comparative biochemistry and physiology 2017 v.211 pp. 49-55
ISSN:
1095-6433
Subject:
abdominal fat, adipose tissue, chicks, crude protein, energy, fatty acid-binding proteins, high fat diet, homeostasis, lipid metabolism, messenger RNA, metabolizable energy, models, neuropeptide Y, peroxisome proliferator-activated receptors, soybean oil, sterols, subcutaneous fat, transcription (genetics)
Abstract:
The purpose of this study was to determine how dietary macronutrient composition and exogenous neuropeptide Y (NPY) affect mRNA abundance of factors associated with lipid metabolism in chick adipose tissue. Chicks were fed one of three isocaloric (3000kcal metabolizable energy (ME)/kg) diets after hatch: high carbohydrate (HC; control), high fat (HF; 30% of ME from soybean oil) or high protein (HP; 25% crude protein). On day 4 post-hatch, vehicle or 0.2nmol of NPY was injected intracerebroventricularly and abdominal and subcutaneous fat depots collected 1h later. In abdominal fat, mRNA abundance of peroxisome proliferator-activated receptor γ (PPARγ) and fatty acid binding protein 4 (FABP4) increased after NPY injection in HF diet-fed chicks. NPY injection decreased expression of PPARγ and sterol regulatory element-binding transcription factor 1 (SREBP1) in the subcutaneous fat of HC diet-fed chicks, whereas SREBP1 expression was increased in the subcutaneous fat of HF diet-fed chicks after NPY injection. An acutely increased central concentration of NPY in chicks affects adipose tissue physiology in a depot- and diet-dependent manner. The chick may serve as a model to understand the relationship between diet and the brain-fat axis' role in maintaining whole body energy homeostasis, as well as to understand metabolic distinctions among fat depots.
Agid:
5704236