PubAg

Main content area

Novel Oleyl Amine-modified Polymannuronic Acid Micelle Loading Tacrolimus for Therapy of Allergic Conjunctivitis

Author:
Cong, Mengyi, Wu, Zhongyu, Xu, Xuelian, Yin, Jiaqi, Li, Hongxia, Zhuang, Bo, Wang, Cheng, Zhao, Xia
Source:
International journal of biological macromolecules 2017
ISSN:
0141-8130
Subject:
animal models, biocompatibility, biodegradability, conjunctivitis, cytotoxicity, drugs, encapsulation, hydrophobicity, medication adherence, micelles, nanocarriers, nanoparticles, nuclear magnetic resonance spectroscopy, particle size, patients, tacrolimus
Abstract:
Marine polysaccharide-based nanomaterial attracted considerable attention due to its biocompatibility and biodegradability. Here, a novel polymannuronic acid derivative (PM-C18) was synthesized as nanocarrier modified with hydrophobic oleyl amine. Its structure was confirmed by Fourier-transformed infrared spectrometry and nuclear magnetic resonance. To investigate its function, we developed tacrolimus (FK506) loaded nanoparticle by self-assembling PM-C18 and studied its drug loading, cytotoxicity and mechanism of in vitro release. Furthermore, we established a mouse model of allergic conjunctivitis (AC) to evaluate the efficacy of FK506 loaded PM-C18. The results indicated that PM-C18 carrier can encapsule FK506 with high drug loading (18%) and average particle size of 110.34±1.6nm. The release mechanism was diffusion initially followed a lasting release induced by swelling and collapse of PM-C18. One-week treatment with FK506 loaded PM-C18 efficiently relieved the symptoms of AC, which indicated it could serve as a potential ophthalmic drug for patients with AC. Compared with FK506 ophthalmic suspension, FK506 loaded PM-C18 improved medication adherence. Most importantly, we developed a novel versatile nanomaterial of PM-C18 which could encapsule other active agents for more applications.
Agid:
5705892