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EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica

Author:
Vandermies, Marie, Denies, Olivia, Nicaud, Jean-Marc, Fickers, Patrick
Source:
Journal of microbiological methods 2017 v.139 pp. 161-164
ISSN:
0167-7012
Subject:
Yarrowia lipolytica, gene editing, yeasts
Abstract:
We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
Agid:
5706135