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A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
- Mansour, Amira, Tammam, Salma, Althani, Asma, Azzazy, Hassan M.E.
- Journal of microbiological methods 2017 v.139 pp. 165-167
- Mycobacterium tuberculosis, nanogold, ribosomal DNA
- The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.