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Comparison of the XTT and resazurin assays for quantification of the metabolic activity of Staphylococcus aureus biofilm

Author:
Alonso, Beatriz, Cruces, Raquel, Pérez, Adrián, Sánchez-Carrillo, Carlos, Guembe, María
Source:
Journal of microbiological methods 2017 v.139 pp. 135-137
ISSN:
0167-7012
Subject:
Staphylococcus aureus, biochemical pathways, biofilm, models
Abstract:
We assessed whether resazurin was as efficient as XTT in the measurement of the metabolic activity of 209 clinical Staphylococcus aureus biofilm using an vitro model comparing the percentage of formazan and resorufin. The overall categorical agreement was 61.2% (r=0.024), which means that resazurin can not substitute XTT.
Agid:
5706145