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Isolation and molecular characterization of Thraustochytrium strain isolated from Antarctic Peninsula and its biotechnological potential in the production of fatty acids

Author:
Caamaño, Esteban, Loperena, Lyliam, Hinzpeter, Ivonne, Pradel, Paulina, Gordillo, Felipe, Corsini, Gino, Tello, Mario, Lavín, Paris, González, Alex R.
Source:
Brazilian journal of microbiology 2017 v.48 no.4 pp. 671-679
ISSN:
1517-8382
Subject:
Thraustochytrium, biofuels, biomass, carbon, dietary supplements, docosahexaenoic acid, eicosapentaenoic acid, genes, geographical distribution, glucose, lipid content, long chain polyunsaturated fatty acids, microbiology, palmitic acid, protists, ribosomal RNA, temperature, Antarctic region
Abstract:
Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16.1–19.7%. On the other hand, long-chain polyunsaturated fatty acids, mainly docosahexaenoic acid and eicosapentaenoic acid are present in a range of 24–48% and 6.1–9.3%, respectively. All factors analyzed in cells (biomass, carbon consumption and lipid content) changed with variations of culture temperature (10°C and 25°C). The growth in glucose at a temperature of 10°C presented the most favorable conditions to produce omega-3fatty acid. This research provides the identification and characterization of a Thraustochytrids strain, with a total lipid content that presents potential applications in the production of nutritional supplements and as well biofuels.
Agid:
5708084