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Novel dendritic structure of alginate hybrid nanoparticles for effective anti-viral drug delivery

Joshy, K.S., George, Anne, Jose, Jiya, Kalarikkal, Nandakumar, Pothen, Laly A., Thomas, Sabu
International journal of biological macromolecules 2017 v.103 pp. 1265-1275
Fourier transform infrared spectroscopy, alginates, antiretroviral agents, cytotoxicity, drug delivery systems, drugs, encapsulation, erythrocytes, ethylene glycol, hemolysis, leukocytes, nanoparticles, transmission electron microscopy
Lipid-polymer hybrid nanoparticles have recently gathered much attention as nanoplatforms for drug delivery applications due to their unique structural properties. In this study zidovudine (AZT) loaded hybrid nanoparticles of alginate (ALG) and stearic acid- poly ethylene glycol (SA-PEG) were synthesized. The structural characterization of drug loaded hybrid nanoparticles were studied using FT-IR spectroscopy, DLS and TEM analysis. These hybrid nanoparticles showed dendritic morphology and it can be used as an efficient carrier for zidovudine. In this drug loaded hybrid system of Alginate –Stearicacid/Poly (ethyleneglycol) Nanoparticles (ASNPs), AZT and alginate form the core wherein SA-PEG forms the external shell. We observed a dendritic morphology with internal voids and channels formed by the core molecule and the external shell forms the closed pack surface groups. The optimized formulation achieved a sub micron size of 407.67±19.18nm with drug encapsulation of 83.18±1.22%, and surface potential of −42.53mV, and has significant stability for six months. Haemolysis and aggregation studies revealed that there were no lysis and aggregation in WBC, RBC and platelets. In-vitro cytotoxicity and cellular uptake of the nanoparticles in Glioma, Neuro2a and Hela cells showed that ASNPs are non toxic. The results indicate that the synthesized hybrid nanoparticles represent a potential carrier for zidovudine, thus possibly increasing zidovudine’s efficiency as an anti-HIV drug.