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Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe3O4@CS/NHCS-Lip as a novel and promising nanobiocatalyst
- Baghban, Ali, Heidarizadeh, Mohammad, Doustkhah, Esmail, Rostamnia, Sadegh, Rezaei, Parisa Fathi
- International journal of biological macromolecules 2017 v.103 pp. 1194-1200
- chemical bonding, chitosan, glutaraldehyde, immobilized enzymes, magnetite, nanocomposites, pancreas, swine, triacylglycerol lipase
- In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite via a novel stepwise dithiocarbamate/chitosan-based method in alternation to glutaraldehyde. Magnetic chitosan nanocomposite was post-modified to produce dithiocarbamate moieties on the surface through amine functions. Then, immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4@CS/NHCS2H via a post-modification. Each step of immobilization was carefully monitored by characterization and all were successfully proved. Comparison of immobilized enzyme with free enzyme showed that the method of immobilization is efficient.