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A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia

Vernon D. Damsteegt, Andrew L. Stone, Oney P. Smith, Lawrence McDaniel, Diana J. Sherman, Chris Dardick, John Hammond, Ramon Jordan, William L. Schneider
Archives of Virology 2013 v.158 pp. 1235-1244
Aphidoidea, Brugmansia candida, Nicotiana tabacum, Potyvirus, antibodies, genome, heat tolerance, host range, inclusion bodies, plant diseases and disorders, plant viruses, sequence analysis, signs and symptoms (plants), tissue analysis, tobacco, trees, virion
A suspected virus disease was identified from an arborescent Brugmansia x candida Pers. (syn. Datura candida Pers.) tree. The causal agent was aphid transmissible at low rates. Viral particles were purified from infected tobacco tissue, analyzed, and purified virions were inoculated into healthy tobacco plants to recreate the symptoms. The virions had a mean length of 720-729 nm, and infected cells contained inclusion bodies typical of potyvirus infections. Analysis of infected tissues and purified virions with a panel of potyvirus-specific antibodies confirmed identification as a potyvirus. Viral host range, dilution end point, thermal tolerance and aphid transmission characteristics were examined. The viral genome (9761 nt) is typical of potyviruses, with the closest related potyvirus being pepper mottle virus, at 72 % nt sequence identity. Based on conventions for naming novel potyviruses, the virus was determined to be a member of a previously undescribed species, tentatively named "Brugmansia mosaic virus" (BruMV).