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Effects of the attachment of small molecules by carbodiimide‐mediated condensation on the physicochemical and functional properties of bovine serum albumin

Murphy, Margaret C, Hoell, Nazlin K
Journal of the science of food and agriculture 1991 v.55 no.1 pp. 117-140
Lewis bases, bovine serum albumin, chemical elements, crosslinking, electrostatic interactions, functional properties, gelation, hens, isoelectric point, lysine, pH, putrescine, titration
The effects of attaching three small molecules, namely N‐butylamide, putrescine and lysine, via the carboxyl groups of bovine serum albumin on its physicochemical and functional properties ere studied. To levels of attachment ere achieved by choice of reaction time. All modified samples had high isoelectric points and ere highly positive hen separated electrophoretically. Partially and extensively modified samples behaved differently in their pH titration curves, their gelation properties and the hipping properties of their lysyl derivatives, in that partially modified samples had high values compared ith the control and extensively modified samples had loer values. This as explicable by to theories: either there is increased electrostatic attraction beteen molecules hose isoelectric points are closer to neutrality, or there is a midpoint here a partial degree of conformational disruption results in superior functional properties. The level of conformational disruption in the extensively modified samples as greater than the optimum, hich resulted in impaired functional properties. An EDC control sample produced ithout added nucleophile as extensively cross‐linked intra‐ or intermolecularly and had impaired functional properties.