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Modulation of in vitro rumen biohydrogenation by Cistus ladanifer tannins compared with other tannin sources

Costa, Mónica, Alves, Susana P, Cabo, Ângelo, Guerreiro, Olinda, Stilwell, George, Dentinho, Maria T, Bessa, Rui JB
Journal of the science of food and agriculture 2017 v.97 no.2 pp. 629-635
Castanea, Cistus ladanifer, biohydrogenation, grape seeds, proanthocyanidins, rumen fluids, sunflower oil, tannins, unsaturated fatty acids
BACKGROUND: Tannins are polyphenolic compounds able to modify the ruminal biohydrogenation (BH) of unsaturated fatty acids, but their activity may vary among different tannin sources. The effect of rockrose (Cistus ladanifer) on BH has never been compared with other more common tannin sources. Tannin extracts (100 g kg⁻¹ substrate dry matter) from chestnut (CH), quebracho (QB), grape seed (GS) and rockrose (CL) were incubated in vitro for 6 h with ruminal fluid using as substrate a feed containing 60 g kg⁻¹ of sunflower oil. A control treatment with no added tannins was also included. RESULTS: Compared with the control, GS and CL, but not CH and QB, increased (P < 0.05) the disappearance of c9,c12‐18:2 with a consequent larger production of c9,t11‐18:2 and t11‐18:1. However, no differences among treatments (P > 0.05) were observed for the disappearance of c9‐18:1 and c9,c12,c15‐18:3. The production of 18:0 was not different (P > 0.05) among treatments, although its proportion in the total BH products was lower (P < 0.05) for GS than for the other treatments. CONCLUSION: Condensed tannins from GS and, to a lesser extent, from CL stimulate the first steps of BH, without a clear inhibition of 18:0 production. © 2016 Society of Chemical Industry