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Colloidal gold‐McAb probe‐based rapid immunoassay strip for simultaneous detection of fumonisins in maize

Yao, Jingjing, Sun, Yaning, Li, Qingmei, Wang, Fangyu, Teng, Man, Yang, Yanyan, Deng, Ruiguang, Hu, Xiaofei
Journal of the science of food and agriculture 2017 v.97 no.7 pp. 2223-2229
cell fusion, corn, cross reaction, fumonisin B1, fumonisin B2, high performance liquid chromatography, immunization, immunoaffinity chromatography, immunoassays, inhibitory concentration 50, monoclonal antibodies, toxicity
BACKGROUND: Fumonisins are a kind of toxic and carcinogenic mycotoxin. A rapid immunochromatographic test strip has been developed for simultaneous detection of fumonisin B₁, B₂ and B₃ (FB₁, FB₂ and FB₃) in maize based on colloidal gold‐labelled monoclonal antibody (McAb) against FB₁ probe. RESULTS: The anti‐FB₁ McAb (2E11‐H3) was produced through immunisation and cell fusion, and identified as high affinity, specificity and sensitivity. The cross‐reaction ratios with fumonisin B₂ and B₃ were accordingly 385% and 72.4%, while none with other analogues. The colloid gold‐labelled anti‐FB₁ McAb probe was successfully prepared and used for establishing the immunochromatographic strip. The test strip showed high sensitivity and specificity, the IC₅₀ for FB₁ was 58.08 ng mL⁻¹, LOD was 11.24 ng mL⁻¹, calculated from standard curve. Moreover, the test strip exhibited high cross‐reactivity with FB₂ and FB₃, and could be applied to the simultaneous detection of FBs (FB₁:FB₂:FB₃ = 12:4:1) in maize sample with high accuracy and precision. The average recoveries of FBs in maize ranged from 90.42% to 95.29%, and CVs were 1.25–3.77%. The results of the test strip for FBs samples showed good correlation with high‐performance liquid chromatography analysis. CONCLUSION: The immunochromatographic test strip could be employed in the rapid simultaneous detection of FB₁, FB₂ and FB₃ in maize samples on‐site. © 2016 Society of Chemical Industry