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Colloidal gold‐McAb probe‐based rapid immunoassay strip for simultaneous detection of fumonisins in maize

Author:
Yao, Jingjing, Sun, Yaning, Li, Qingmei, Wang, Fangyu, Teng, Man, Yang, Yanyan, Deng, Ruiguang, Hu, Xiaofei
Source:
Journal of the science of food and agriculture 2017 v.97 no.7 pp. 2223-2229
ISSN:
0022-5142
Subject:
cell fusion, corn, cross reaction, fumonisin B1, fumonisin B2, high performance liquid chromatography, immunization, immunoaffinity chromatography, immunoassays, inhibitory concentration 50, monoclonal antibodies, toxicity
Abstract:
BACKGROUND: Fumonisins are a kind of toxic and carcinogenic mycotoxin. A rapid immunochromatographic test strip has been developed for simultaneous detection of fumonisin B₁, B₂ and B₃ (FB₁, FB₂ and FB₃) in maize based on colloidal gold‐labelled monoclonal antibody (McAb) against FB₁ probe. RESULTS: The anti‐FB₁ McAb (2E11‐H3) was produced through immunisation and cell fusion, and identified as high affinity, specificity and sensitivity. The cross‐reaction ratios with fumonisin B₂ and B₃ were accordingly 385% and 72.4%, while none with other analogues. The colloid gold‐labelled anti‐FB₁ McAb probe was successfully prepared and used for establishing the immunochromatographic strip. The test strip showed high sensitivity and specificity, the IC₅₀ for FB₁ was 58.08 ng mL⁻¹, LOD was 11.24 ng mL⁻¹, calculated from standard curve. Moreover, the test strip exhibited high cross‐reactivity with FB₂ and FB₃, and could be applied to the simultaneous detection of FBs (FB₁:FB₂:FB₃ = 12:4:1) in maize sample with high accuracy and precision. The average recoveries of FBs in maize ranged from 90.42% to 95.29%, and CVs were 1.25–3.77%. The results of the test strip for FBs samples showed good correlation with high‐performance liquid chromatography analysis. CONCLUSION: The immunochromatographic test strip could be employed in the rapid simultaneous detection of FB₁, FB₂ and FB₃ in maize samples on‐site. © 2016 Society of Chemical Industry
Agid:
5714060